Registro completo |
Provedor de dados: |
PAB
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País: |
Brazil
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Título: |
Viability of dourado embryos cooled in different cryoprotectant solutions
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Autores: |
Fornari,Darci Carlos
Streit Junior,Danilo Pedro
Galo,Juliana Minardi
Guerreiro,Luís Jayme
Povh,Jayme Aparecido
Barrero,Nelson Mauricio Lopera
Oliveira,Diego de
Ribeiro,Ricardo Pereira
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Data: |
2013-08-01
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Ano: |
2013
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Palavras-chave: |
Salminus brasiliensis
Cryopreservation
Fish embryo
Embryo preservation
Larvae eclosion
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Resumo: |
The objective of this work was to evaluate the effect of different cryoprotectants on the viability of dourado (Salminus brasiliensis) embryos. Ten cryoprotectant solutions were tested. For each solution, 300 embryos were selected at the closing of the blastopore stage, and 300 more embryos were used as a negative control. After cooling (-8ºC for 6 hours), the embryos were rehydrated directly in the incubator until hatching. The best result is obtained with the cryoprotectant solution containing 9% methanol associated with 17% sucrose, resulting in a larvae hatching rate of 67.06%.
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Tipo: |
Info:eu-repo/semantics/report
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Idioma: |
Inglês
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Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000800060
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Editor: |
Embrapa Secretaria de Pesquisa e Desenvolvimento
Pesquisa Agropecuária Brasileira
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Relação: |
10.1590/S0100-204X2013000800057
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Formato: |
text/html
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Fonte: |
Pesquisa Agropecuária Brasileira v.48 n.8 2013
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Direitos: |
info:eu-repo/semantics/openAccess
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